(111)Indium-oxine labelling for evaluating the homing process of autologous osteoblasts implanted percutaneously in atrophic nonunion fractures.
Identifieur interne : 000921 ( Main/Exploration ); précédent : 000920; suivant : 000922(111)Indium-oxine labelling for evaluating the homing process of autologous osteoblasts implanted percutaneously in atrophic nonunion fractures.
Auteurs : RBID : pubmed:23180104English descriptors
- KwdEn :
- Adolescent, Adult, Cell Proliferation, Cell Survival, Cells, Cultured, Female, Fracture Healing (physiology), Fractures, Ununited (therapy), Humans, Indium Radioisotopes (diagnostic use), Male, Middle Aged, Organometallic Compounds, Osteoblasts (transplantation), Oxyquinoline (analogs & derivatives), Prospective Studies, Radiopharmaceuticals (diagnostic use), Transplantation, Autologous.
- MESH :
- chemical , analogs & derivatives : Oxyquinoline.
- chemical , diagnostic use : Indium Radioisotopes, Radiopharmaceuticals.
- physiology : Fracture Healing.
- therapy : Fractures, Ununited.
- transplantation : Osteoblasts.
- Adolescent, Adult, Cell Proliferation, Cell Survival, Cells, Cultured, Female, Humans, Male, Middle Aged, Organometallic Compounds, Prospective Studies, Transplantation, Autologous.
Abstract
The aim of the study was to control the in vivo localisation of implanted cells in cell-based therapies. Labelling cells with (111)indium-oxine is one of the most interesting methods proposed. We evaluated this method in the setting of autologous osteoblast implantation in nonunion fractures.
DOI: 10.1007/s00264-012-1719-y
PubMed: 23180104
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">(111)Indium-oxine labelling for evaluating the homing process of autologous osteoblasts implanted percutaneously in atrophic nonunion fractures.</title><author><name sortKey="Hauzeur, Jean Philippe" uniqKey="Hauzeur J">Jean-Philippe Hauzeur</name><affiliation wicri:level="1"><nlm:affiliation>Department of Rheumatology, University of Liège, Liège, Belgium. jean-philippe@hauzeur.org</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Rheumatology, University of Liège, Liège</wicri:regionArea></affiliation></author><author><name sortKey="Bernard, Claire" uniqKey="Bernard C">Claire Bernard</name></author><author><name sortKey="Egrise, Dominique" uniqKey="Egrise D">Dominique Egrise</name></author><author><name sortKey="Kurth, William" uniqKey="Kurth W">William Kurth</name></author><author><name sortKey="Van Cauwenberge, Henry" uniqKey="Van Cauwenberge H">Henry Van Cauwenberge</name></author><author><name sortKey="Lechanteur, Chantal" uniqKey="Lechanteur C">Chantal Lechanteur</name></author><author><name sortKey="Gillet, Philippe" uniqKey="Gillet P">Philippe Gillet</name></author><author><name sortKey="Beguin, Yves" uniqKey="Beguin Y">Yves Beguin</name></author><author><name sortKey="Malaise, Michel" uniqKey="Malaise M">Michel Malaise</name></author><author><name sortKey="Hustinx, Roland" uniqKey="Hustinx R">Roland Hustinx</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="doi">10.1007/s00264-012-1719-y</idno><idno type="RBID">pubmed:23180104</idno><idno type="pmid">23180104</idno><idno type="wicri:Area/Main/Corpus">000927</idno><idno type="wicri:Area/Main/Curation">000927</idno><idno type="wicri:Area/Main/Exploration">000921</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Cell Proliferation</term><term>Cell Survival</term><term>Cells, Cultured</term><term>Female</term><term>Fracture Healing (physiology)</term><term>Fractures, Ununited (therapy)</term><term>Humans</term><term>Indium Radioisotopes (diagnostic use)</term><term>Male</term><term>Middle Aged</term><term>Organometallic Compounds</term><term>Osteoblasts (transplantation)</term><term>Oxyquinoline (analogs & derivatives)</term><term>Prospective Studies</term><term>Radiopharmaceuticals (diagnostic use)</term><term>Transplantation, Autologous</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Oxyquinoline</term></keywords><keywords scheme="MESH" type="chemical" qualifier="diagnostic use" xml:lang="en"><term>Indium Radioisotopes</term><term>Radiopharmaceuticals</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fracture Healing</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Fractures, Ununited</term></keywords><keywords scheme="MESH" qualifier="transplantation" xml:lang="en"><term>Osteoblasts</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Cell Proliferation</term><term>Cell Survival</term><term>Cells, Cultured</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Organometallic Compounds</term><term>Prospective Studies</term><term>Transplantation, Autologous</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of the study was to control the in vivo localisation of implanted cells in cell-based therapies. Labelling cells with (111)indium-oxine is one of the most interesting methods proposed. We evaluated this method in the setting of autologous osteoblast implantation in nonunion fractures.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23180104</PMID><DateCreated><Year>2012</Year><Month>12</Month><Day>31</Day></DateCreated><DateCompleted><Year>2013</Year><Month>05</Month><Day>27</Day></DateCompleted><DateRevised><Year>2014</Year><Month>01</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-5195</ISSN><JournalIssue CitedMedium="Internet"><Volume>37</Volume><Issue>1</Issue><PubDate><Year>2013</Year><Month>Jan</Month></PubDate></JournalIssue><Title>International orthopaedics</Title><ISOAbbreviation>Int Orthop</ISOAbbreviation></Journal><ArticleTitle>(111)Indium-oxine labelling for evaluating the homing process of autologous osteoblasts implanted percutaneously in atrophic nonunion fractures.</ArticleTitle><Pagination><MedlinePgn>131-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00264-012-1719-y</ELocationID><Abstract><AbstractText Label="PURPOSE" NlmCategory="OBJECTIVE">The aim of the study was to control the in vivo localisation of implanted cells in cell-based therapies. Labelling cells with (111)indium-oxine is one of the most interesting methods proposed. We evaluated this method in the setting of autologous osteoblast implantation in nonunion fractures.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">An in vitro study of osteoblasts was conducted after (111)indium-oxine labelling. Radioactivity retention and viability, proliferation and the ability to produce alkaline phosphatase were evaluated in a seven-day culture. In vivo labelling of implanted osteoblastic cells was conducted during a therapeutic trial of atrophic nonunion fractures, with the leakage outside the nonunion site and local uptake evolution at four, 24 and 48 hour being studied.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The mean labelling efficiency for osteoprogenitors was 78.8 ± 4.6 %. The intracellular retention was 89.4 ± 2.1 % at three hours and 67.3 ± 4.7 % at 18 hours. The viability assessed at three hours was 93.7 ± 0.6 %. After seven days of culture, morphology and alkaline phosphatase staining were similar for both labelled and unlabelled control cells, although the proliferation rate was decreased in the labelled cells. Some local intraosseous leakage was observed in four of 17 cases. All patients showed uptake at the injection site, with four having no other uptake. Four patients showed additional uptake in the bladder, liver and spleen, while 11 patients had additional uptake in the lungs in addition to the bladder, liver and spleen. The activity ratios (injection site/body) were 48 ± 28 % at four hours, 40 ± 25 % at 24 hours and 35 ± 25 % at 48 hours. After correcting for decay, the activity within the injection site was 82 ± 15 % at 24 hours and 69 ± 11 % at 48 hours compared with the activity measured at four hours. No relationship was found between uptake and radiological bone repair.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The (111)indium-oxine labelling appears to be a good method for monitoring the behaviour of the osteoblastic cells after their implantation in atrophic nonunion fractures.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hauzeur</LastName><ForeName>Jean-Philippe</ForeName><Initials>JP</Initials><Affiliation>Department of Rheumatology, University of Liège, Liège, Belgium. jean-philippe@hauzeur.org</Affiliation></Author><Author ValidYN="Y"><LastName>Bernard</LastName><ForeName>Claire</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Egrise</LastName><ForeName>Dominique</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Kurth</LastName><ForeName>William</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Van Cauwenberge</LastName><ForeName>Henry</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Lechanteur</LastName><ForeName>Chantal</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Gillet</LastName><ForeName>Philippe</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Beguin</LastName><ForeName>Yves</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Malaise</LastName><ForeName>Michel</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Hustinx</LastName><ForeName>Roland</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>11</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Int Orthop</MedlineTA><NlmUniqueID>7705431</NlmUniqueID><ISSNLinking>0341-2695</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indium Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Organometallic Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Radiopharmaceuticals</NameOfSubstance></Chemical><Chemical><RegistryNumber>14514-42-2</RegistryNumber><NameOfSubstance>indium oxine</NameOfSubstance></Chemical><Chemical><RegistryNumber>5UTX5635HP</RegistryNumber><NameOfSubstance>Oxyquinoline</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Nucl Med Commun. 2009 Mar;30(3):210-6</RefSource><PMID Version="1">19262283</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Bone. 2011 Nov;49(5):1005-9</RefSource><PMID Version="1">21821156</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Br J Haematol. 1999 Dec;107(4):895-902</RefSource><PMID Version="1">10606901</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Bone Joint Surg Am. 2004 Jun;86-A(6):1153-60</RefSource><PMID Version="1">15173287</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Nucl Med. 1976 Jun;17(6):488-92</RefSource><PMID Version="1">1262966</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Eur J Nucl Med Mol Imaging. 2007 May;34(5):715-21</RefSource><PMID Version="1">17096094</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Acta Oncol. 1996;35(7):947-52</RefSource><PMID Version="1">9004776</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Eur J Nucl Med. 1997 May;24(5):497-504</RefSource><PMID Version="1">9142729</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Injury. 2005 Nov;36 Suppl 4:S30-7</RefSource><PMID Version="1">16291321</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Eur J Nucl Med Mol Imaging. 2006 Oct;33(10):1171-7</RefSource><PMID Version="1">16763813</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Clin Rheumatol. 1986 Sep;5(3):346-58</RefSource><PMID Version="1">3780142</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Proliferation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Survival</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Fracture Healing</DescriptorName><QualifierName MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Fractures, Ununited</DescriptorName><QualifierName MajorTopicYN="Y">therapy</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium Radioisotopes</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Organometallic Compounds</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Osteoblasts</DescriptorName><QualifierName MajorTopicYN="Y">transplantation</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Oxyquinoline</DescriptorName><QualifierName MajorTopicYN="N">analogs & derivatives</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Prospective Studies</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Radiopharmaceuticals</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Transplantation, Autologous</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC3532634</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>10</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>11</Month><Day>7</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2012</Year><Month>11</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>11</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>11</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>5</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1007/s00264-012-1719-y</ArticleId><ArticleId IdType="pubmed">23180104</ArticleId><ArticleId IdType="pmc">PMC3532634</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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